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Image Search Results
Journal: bioRxiv
Article Title: Genetic Encoding of A Nonhydrolyzable Phosphotyrosine Analog in Mammalian Cells
doi: 10.1101/2021.11.30.470659
Figure Lengend Snippet: a Western blot of cell lysates expressing STAT1 variants. Lanes 1 and 5, wild-type STAT1-EGFP without IFN-γ induction; lanes 2 and 6, wild-type STAT1-EGFP with IFN-γ induction; lanes 3 and 7, STAT1-701TAG-EGFP without CMF; lanes 4 and 8, STAT1-701TAG-EGFP with CMF. Lane M is the molecular weight marker. The calculated molecular weight of STAT1-EGFP variants was 115.9 kDa. Lanes 1-4 were detected by phospho-STAT1 (pTyr701) antibody. Lanes 5-8 were detected by anti-STAT1 antibody. b Images of cells expressing STAT1 variants. Left panel 293T-ΔSTAT1 cells; mid-left panel, 293T-ΔSTAT1 cells expressing wild-type STAT1; mid-right panel, 293T-ΔSTAT1 cells expressing STAT1-701TAG and the wild-type EcTyrRS; right panel, 293T-ΔSTAT1 cells expressing STAT1-701TAG and the CMFRS. Immunofluorescence images were obtained by the treatment of permeabilized cells with phospho-STAT1 (pTyr701) antibody followed by anti-rabbit secondary antibody-Alex594 conjugate. Scale bars, 50 μm.
Article Snippet: Primary antibodies used in this study included
Techniques: Western Blot, Expressing, Molecular Weight, Marker, Immunofluorescence